Single-Use Centrifuges
Benefits of Single-Use Systems
Optimize Product Recovery and Scalability
Since 2009, CARR Biosystems has been providing single-use separation systems to biopharma companies seeking low-shear separation, high-recovery performance, and fast processing time. Avoid the expense and waste of CIP/SIP stainless steel centrifuges and step up to the scalable, industry-leading, UniFuge platform.
More Data in Less Time
- Get more data in less time while lowering costs. Our smallest scale UniFuge technology - UFMicro - requires minimal cell culture volume and technician time.
- Optimize your process performance when developing process CTQs with low-shear separation and remarkably high processing speed.
Scale-up Seamlessly with UniFuge
- De-risk Process Scaling: Establish easily transferable CTQs as you pass clinical milestones.
- Optimize product quality via tunable process settings (flow, force).
- Processing volumes to support you from process development to commercial manufacturing:
| UFMicro | UFMini | Pilot Shallow | Pilot Standard | U2K | |
|---|---|---|---|---|---|
| Min Proc Vol | 70 ml | 270 ml | 1 L | 1.8 L | 10 L |
| Max Proc Vol | 25 L | 25 L | 500 L | 500 L | 2000+ L |
Unsurpassed Performance
- Optimize separation performance with best-in-class low-shear separation ensuring:
- High cell viability for cell therapy and cellular agriculture applications
- Isolation of antibodies from cell culture supernatant
- Reduction of BSA levels well below the required minimum
- Achieve high processing speeds without sacrificing the separation efficiency needed for demanding applications
With a small footprint, UniFuge systems are easy to operate, simplify training and require fewer staff than traditional centrifuges.
- Low shear
- Clear center
- Reduce filtration needs by 85%
- Set up and change over within 15 min
- Cell viability up to 98%
- Yield up to 98%
- Confidently scale up to the U2k® or scale down to the UFMini®
- Variable speed up to 4,000 x g
- Reduce HCP and other contaminants
- GMP-compliant data trail 21cfrPart11
- Extractables data available
Publications
Want to learn more about the performance of single-use centrifuges from CARR Biosystems? Review whitepapers, case studies, and more here.
Key Features
Advantages of Single-Use Centrifuges
Easy-to-use, reliable equipment that delivers high product recovery.
Performance
Our proven low-shear technology boasts up to 98% yield and 98% or higher typical cell viability.
Scalability
Our equipment can be used from development to commercial licensure and across different reactor volumes.
Ease of Use
Our machines are rapid to install and simple to operate.
Small Footprint
Our single-use centrifuges have a small footprint.
The UniFuge Family
Our Single-Use Centrifuges
The scalable UniFuge family of centrifuges has a variable g-force up to 4,000 x g. They employ gamma-irradiated sterile single-use modules, reducing the risk of contamination.
UniFuge® Pilot
Single-Use System
The UniFuge® Pilot uses a gamma-irradiated, single-use module to eliminate cleaning needs. It is designed for primary clarification of mammalian cell cultures (e.g., CHO cells), and harvesting (including washing and concentrating) stem cells and other types of cells for biotechnology and pharmaceutical applications.
- Bowl capacity: 1.8 L
- Flow rate from 1-4 L/min (higher flow rates may be achieved, based on application)
- Small bench top footprint: 61 cm wide x 99 cm deep x 117 cm high (24” x 39” x 46”)
- Easy to handle: Total weight ~ 291kg (640 lbs) - Easily movable with stainless steel casters leveling on skid with vibration isolators
- Gamma-irradiated single-use modules
- Scale processes up with the U2k® or down with the UFMini®
Small Footprint
The UniFuge has a small footprint.
Confidently Scale Up, Down or Out
The accurate transfer of operating protocols to the UFMini® or U2k® is streamlined as you move from early-stage development to commercial manufacturing and licensure.
Streamline Processes
Uses a sterile single-use module that is easy to install and replace after each run.
Performance
Low-shear centrifugation allows for high-quality and high-recovery separation.
Save Time
Easily incorporated into a fully-automated workflow and significantly reduces processing time.
Efficiency
Software and automation minimize process upsets and errors.
Minimize Contamination Risks
This single-use system reduces contamination risks. It provides flexibility for multi-product facilities.
Stop-N-Go
The UniFuge systems are designed to resume and recover from an interrupted process without product loss.
Cell Washing
The UniFuge systems can hold materials in the bowl for washing and buffer exchanges.
Reliable
Built in a state-of-the art U.S. facility, following the highest standards of quality control.
Instrument
- Bowl capacity: 1.8 L
- Flow rate from 1-4 L/min (higher flow rates may be achieved, based on application)
- Processing up to 4 L/min
- Variable speed from 300 to 4,000 x g
- Integrated trolley
- Designed for use at room temperatures (jackets available)
- Optional data acquisition for SCADA System via Ethernet connection
- cGMP-compliant 21 CFR Part 11 data trail
- Low noise level (below 80 dB) during operation
- Instrument built in a state-of-the-art ISO 9001 US facility, following the highest standards of quality control.
- Fully-automated, intuitive software
- PLC color touch screen
Single-use Modules
- Lightweight single-use module with integral tube set
- Predictable supply chain with multiple manufacturing facilities in the United States
- Gamma-irradiated
- Extractables data available
- Compliant with ISO 11137 to SAL 10-6
- Continuous discharge of supernatant and intermittent discharge of cell culture
- Modules can be tube welded to single-use bioreactor connections (customer-specified single-use connectors are available upon request)
- Reduce HCP and other contaminants
How It Works
The UniFuge is completely automated with flexible cycle parameter entry. The feed suspension is gently pumped to the module and the cells settle to the outer radius while the clear supernatant is continuously discharged. Once the module has filled with cells, the controller stops the rotor and discharges the cells. This cycle is repeated until the bioreactor volume has been processed.
Automation and General Specifications
- Total skid weight of 291 kg (690 lbs)
- Allen-Bradley® PLC
- Maximum case pressure (for cooling) 6.9 bar (100 psi)
- Center feed for low shear operation
- Concentric tubes to introduce feed and remove supernatant
- PCV up to 25%
Utility Requirements
- Power configurations: 120/230 VAC, 50/60 Hz, 1 Ph, 10 A
- Optional: 120 VAC, 50/60 Hz, 1 Ph, 20 A
- Pneumatic: Clean, dry air supply 4.5-6.6 bar (65-95 PSI), 57 SLPM (2 SCFM)
Single-Use Module
- 1800 mL single-use modules with 12 mm (1/2”) ID x 19 mm (3/4”) OD Silicone tubing
- Continuous discharge of supernatant and intermittent discharge of cell culture
- Components compliant with physiochemical tests: intermittent discharge of cell culture: USP <87> <88> Class VI, USP 661 (or ISO 10993) for plastic materials
- Center feed for low-shear operation
- Concentric tubes to introduce feed and remove supernatant
- This one is perfect...
- The UniFuge is scalable, easier to operate, and my process yield is better than using depth filters
- The UniFuge had the shortest processing time...
- The UniFuge has higher g forces and flow rates...
- The UniFuge was fairly simple to use and scaled nicely from the UFMini
- The UniFuge line is easy to scale-up to the U2k or down to the UFMini
Endorsements
See What People Are Saying About Our Equipment
“The U2k was fairly simple to use and scales nicely from the UFMini and UniFuge.”
Cell Therapies Bioproduction Expert
“This really opens the door with the potential to go above 300 L.”
mRNA and pDNA Bioproduction Expert
“The 20L/min flow rate is great for harvesting from 1,000-2,000 L+ bioreactors. Processing ~1,000 L/hr is very helpful for our operators. It would save a significant amount of time.”
mAb Bioproduction Expert
“The UniFuge line is easy to scale-up to the U2k or down to the UFMini.”
mAbs Bioproduction Expert
“The UniFuge was fairly simple to use and scaled nicely from the UFMini.”
Cell Therapies Bioproduction Expert
“The UniFuge has higher g forces and flow rates [compared to competitive offerings].”
Viral Vector Bioproduction Expert, HEK
“The UniFuge had the shortest processing time versus Ksep.”
Protein Separation Bioproduction Expert, CHO
"The UniFuge is scalable, easier to operate, and my process yield is better than using depth filters."
Protein Separation Bioproduction Expert, CHO
"This one is perfect. This would reduce our harvest time significantly. I think it fits well into the needs for a Phase III trial."
Cell Therapy Bioproduction Expert
“[The UniFuge line] is scalable, easier to operate, and my process yield is better than using depth filters.”
Protein Separation Bioproduction Expert, CHO
“The UniFuge had the shortest processing time versus Ksep.”
Protein Separation Bioproduction Expert, CHO
“The UniFuge line is simpler and required less calibration than other solutions we have tried.”
Protein Separation Bioproduction Expert, CHO
“The UniFuge was easy to scale-up, and the Viafuge enables us to recover viable cells at large scale, beyond what single-use can typically accomplish."
Cellular Agriculture Entrepreneur
“My biggest reason to avoid centrifuges has been that they don’t scale down, so process development is hard, and clean-in-place is a pain. [UniFuge] neatly addresses both.”
Viral Vector Gene Therapy Bioproduction Expert
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