Viafuge ® V12

Product Overview

Clean-in-Place / Sterilize-in-Place System

The Viafuge® V12 is designed for fast, gentle medium- to large-scale cell harvesting and clarification. Capable of separating a wide variety of mammalian and insect cells with little to no damage, the Viafuge V12 offers high purity results with undetectable cell concentrations in clarified supernatant.

Bowl capacity: 9.6 L or 16.7 L
Flow rates up to 2400 L/hr for batches up to 2000 L
Footprint: ~ 127 cm wide x 249 cm deep x 287 cm high (50” x 98 ” x 113”)
Total weight ~ 1119 kg (2,460 lbs)
Gentle cell harvesting and supernatant recovery
scalableCan be scaled-down to smaller automated Pilot model
Improved product processing
Concentrated cells and supernatant can be recovered without breaking containment
20-fold concentration factor

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Low-Shear Performance

The Viafuge V12’s unique design minimizes shear forces on cells, boasting a typical cell viability of 98% or higher and up to 98% yield.

Scalability

Processes developed on the Viafuge® Pilot can be seamlessly scaled to the Viafuge V12.

Features

Bowl capacity: 9.6 L or 16.7 L
Flow rates up to 2400 L/hr for batches up to 2000 L
Variable speeds up to 5000 x g
Sealed automatic operation
Fully-automated operation
Closed-system design with CIP/SIP
cGMP construction
ASME-rated designs available
Flexible optimization
Optional Hazardous Duty (Class 1, Div 1) CE configurations are available
State-of-the-art manufacturing facility located in the United States

How It Works

Using an innovative design, the Viafuge V12 introduces feed through a stationary pipe, where it is accelerated to full rotational speed under low shear before entering the titanium-alloy bowl. Cells are then concentrated along a gentle path between the core and the bowl wall. The clarified supernatant is continuously discharged by gravity at atmospheric pressure. Periodically, the concentrated cells are automatically recovered from the Viafuge V12 for collection, and the cycle is repeated to process the remainder of the feed batch.

Automation and General Specifications

Allen Bradley® or Siemens® PLC
Allen Bradley® or Siemens® HMI touchscreen 30.7 cm (12.1”)
Maximum case pressure (for cooling) 6.9 bar (100 psi)

Utility Requirements

Power configuration: Three phase voltage supply:

US: 230V/60 Hz, 20 A
Europe: 200V/50 Hz, 20 A
Japan: 200V/60 Hz/50 Hz, 20 A

Optional: Others as needed
Pneumatic: Clean, dry air supply 6 bar (90 psi) at 1 SLPM

Process Wetted Parts Construction

USP Class VI components

316L Stainless Steel
17-4 PH Stainless Steel
Nitronic 60 Stainless Steel
Platinum cured silicone hoses
EPDM elastomers
Ti-6Al-4V Titanium

Stainless Steel Centrifuges Brochure

Endorsements

See What People Are Saying About Our Equipment

“The UniFuge was easy to scale-up, and the Viafuge enables us to recover viable cells at large scale, beyond what single-use can typically accomplish."

Cellular Agriculture Entrepreneur

“We replaced our bottle centrifuges with a single continuous Viafuge, saving cleaning, autoclaving, and manual manipulations."

Animal Vaccine Manufacturing Operations Manager

"Gentle handling of our cells at scale resulted in higher VLP recovery.”

Director of Operations, Vaccine Manufacturing

“The high g-force of the Powerfuge [e. Coli lysate clarification] reduced our downstream clarification needs.”

Senior Process Development Scientist, pDNA

“Removing large particles with Powerfuge reduced the number of filtration steps.”

Manufacturing Engineer, Pigments

“The Powerfuge closed up our ethanol process and its automatic scraping improved our recovery.”

Associate Director of Vaccine Franchise

“The explosion-proof Powerfuge closed up our solvent process for a safe and environmentally friendly manufacturing method.”

Senior Process Engineer, Pigments