Publications

How Centrifugation is Keeping Pace with Bioprocessing Needs: Tunable Cell Processing Solutions Ensure Separation Efficiency

Case Study: Cultured Meat Using Single-Use Centrifugation

Cultivated meat cells can be collected with single-use centrifuges including the UFMini, UniFuge Pilot, and the U2k. The CARR Biosystems tubular bowl design offers several advantages. Download this case study to learn more.

Achieving Optimal Cell Harvest for Allogeneic Therapies with Purpose-Built Harvest Technologies

Cell harvesting is crucial to the cell therapy manufacturing process, bridging the gap between cells’ optimized growth environment and a final formulation that retains their potency and efficacy. The number of variables that impact harvesting, coupled with the complexities introduced by the unique CQAs of each cell therapy and the fragility of many emerging cell types, can result in a challenging balancing act that requires significant expertise to navigate.

The Shear Problem Facing Cell Separation

Efficient FBS Removal and Cell Concentration in Vero Cell Cultures

The study explored the application of the CARR Biosystem’s UFMini to achieve concentration and FBS removal from Vero cell cultures using PBS as the wash solution. The UFMini’s capability to concentrate and wash Vero cells efficiently while preserving their viability in a closed, automated system underscores its potential for optimizing Vero cell culture processes.

Clarification of HEK293 Cells Using Centrifugation: Results from the UniFuge® Pilot and the UFMini

This case study presents data from the CARR Biosystems team highlighting the UniFuge family and the ability to clarify HEK293 cells in a manner yielding better results than other forms of filtration.

Single-Use Systems Boost Viral Titer from HEK293 Cell Culture

Depth filtration has become a trusted standby for scientists and engineers looking to separate viral vectors – a popular delivery system for gene-based therapies and vaccines – from the HEK293 cell culture in which they’re typically grown. But as emerging therapies advance from the lab and into the clinic, and viral vector production demands rise to fuel these therapies, relying on depth filtration alone to clarify HEK293 cell culture and collect viral titer becomes too labor-intensive, costly, and inefficient to make for a seamless scaling-up transition. In this case study, we show how the UFMini single-use centrifuge significantly reduces filtration needs and drastically enhances the amount of viral titer collected when compared to conventional methods.

Primary Clarification of Lentiviral Vector with Low-Shear Separation

Lentiviral vectors are commonly used in the production of ex vivo cell therapies to transduce various cell types. Lentiviral vectors are sensitive to the shear forces of traditional continuous centrifugation systems. Additionally, significant yield losses due to adsorption effects can be seen during the primary clarification of lentiviral vectors with single-use filtration methods such as depth filtration.